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How to Request TMEN Resources

If you are interested in obtaining any of the listed TMEN resources, please complete the following request form and email it to:

Christine Siemon, Division of Cancer Biology, NCI at siemonc@mail.nih.govTMEN Reagent Request Form.doc

TMEN Resources: 

EHS (Engelbreth-Holm-Swarm) sarcoma-derived laminin rich matrix

TMEN has developed a pool of EHS sarcoma cell-derived extracellular matrix for distribution to the cancer research community who work on three-dimensional models to study tumor-host interactions.

Novel antibodies to detect cancer stem cells and stromal cells

This resource consists of validated commercially available antibody reagents for cancer stem cells and stromal cell-specific genes or proteins (biomarkers) that are critical for studying tumor-host interactions.

Human xenograft tumor bank with characterized stem cell populations

A tumor bank of characterized human breast and colon solid tumors containing xenografts has been established. Vials of frozen tumor cells from each tumor type have been stored for use. Recipients would be responsible for amplifying the stem cells and serving as a central bank for their institution as well as other investigators outside their institution. 

Bone Marrow Derived Cells from donor mice

The BMDC bank contains bone marrow cells from C57BL/6J and C57BL/6-Tg-(UBC-GFP) 30 Scha/J mouse lines in cryopreserved aliquots that can be re-infused as needed.

RCAS Constructs

RCAS(A)-GFP – This is a avian retroviral vector for GFP expression in TVa mice. The vector needs to be introduced in DF1 cells for virus generation. Normally the DF1 cells themselves are introduced into mice directly.

RCAS(B)-DsRed - This is a avian retroviral vector for DsRed expression in TVb mice. The vector needs to be introduced in DF1 cells for virus generation. Normally the DF1 cells themselves are introduced into mice directly.

For more information, click here.

Other DCB Programs with Research Resources